McAbGB2识别的乳腺癌血清抗原性质研究

对抗人乳腺癌血清抗原单克隆抗体GB₂（McAbGB₂）识别的抗原（McAbGB₂抗原）性质及含量进行了研究．结果表明，McAbGB₂抗原是由糖及蛋白质组成的复合蛋白质，不耐热；McAbGB₂抗原决定簇不存在于铁蛋白及癌胚抗原；蛋白质印边检测表明McAbGB₂抗原有两条区带，分子量分别为116及45ku，分布于血液及癌组织；用McAbGB₂进行血清学分析（ELISA试验），结果表明：乳腺癌阳性符合率达98％（50／51），正常人及乳腺良性肿瘤病人的假阳性率分别为6％（3／50）及6．7％（1／15）．McAbGB₂抗原可能是新的乳腺癌相关抗原．

A Study on Character of Serum Antigen Recognized by McAbGB2 in Breast Cancer

A study on character and content of antigen recognized by McAbGB₂ against serum antigen of human breast cancer was reported.The results demonstrated that the antigen recognized by McAbGB₂ is a complex protein which is composed of carbohydrate and protein and it is susceptible to heat. Competitive ELISA test showed that the binding of McAbGB₂ to its antigen could not be inhibited by ferritin and CEA. Western blot revealed that McAbGB₂ recognized antigen with molecular weight of 116 and 45 ku. The antigen recognized by McAbGB₂ exists in breast cancer tissue and serum. ELISA test with McAbGB₂ showed the rate of accord with positivity is 98 %(50/51) in breast cancer patient sera and the rates of false positivity are 6 % (3/50) in normal serum samples tend 6.7% (1/5)in benign breast disease sera respectively. The antigen recognized by McAbGB₂ may be a new tumor-associated antigen.