| 一种鉴定过氧化氢酶活性的铁染色法 |
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| 引用本文： | 董泗建,刘昌玲. 一种鉴定过氧化氢酶活性的铁染色法[J]. 生物化学与生物物理进展, 1996, 23(1): 86-88 |
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| 作者姓名： | 董泗建 刘昌玲 |
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| 作者单位： | 军事医学科学院毒物药物研究所;军事医学科学院毒物药物研究所 |
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| 基金项目： | 总后“八五”招标课题项目. |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳(PAGE)技术，建立了一种新的鉴定过氧化氢酶活性的方法──铁染色法．结果表明，细菌过氧化氢酶及牛肝过氧化氢酶显示单一的酶活性带．该方法操作简单、快速、灵敏度高、专一性强.是一种切实可行的鉴定过氧化氢酶活性染色法.
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| 关 键 词： | 过氧化氢酶 聚丙烯酰凝胶电泳 铁染色法 |
| 收稿时间： | 1995-03-24 |
| 修稿时间： | 1995-08-14 |
An Iron Staining Method for Determination of Catalase Activity |
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| Dong Sijian and Liu Changling. An Iron Staining Method for Determination of Catalase Activity[J]. Progress In Biochemistry and Biophysics, 1996, 23(1): 86-88 |
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| Authors: | Dong Sijian and Liu Changling |
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| Affiliation: | Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences;Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences |
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| Abstract: | An iron staining method for the determination of catalase activity based on the polyacrylamide gel electrophoresis technique was established. The results indicated thatboth bacterial catalase and bovine livercatalase showed unique band in the gel. This staining method was convenient, quick and sensitive for the measurement of catalase activity. |
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| Keywords: | catalase polyacrylamide gel electrophoresis iron staining |
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