氧化修饰脂蛋白刺激人动脉平滑肌细胞DNA合成

动脉平滑肌细胞(SMC)是动脉粥样硬化(As)斑块中的主要细胞, 它的增殖在As的形成过程中极为重要. 在建立人主动脉SMC体外培养法的基础上, 通过 ³H-TdR掺入实验观察了人低密度脂蛋白(LDL)、极低密度脂蛋白(VLDL)及高密度脂蛋白(HDL)和相应的氧化修饰型脂蛋白对培养人SMC DMA合成的影响.结果发现，HDL对 ³H-TdR掺入SMC DNA无影响(P>0.05); LDL和VLDL ³H-TdR掺入量明显增加(P<0.05);OX-LDL, OX-VLDL及OX-HDL均使 ³H-TdR掺入DNA显著增加(P<0.01).结果表明，LDL和OX-LDL, OX-VLDL及OX-HDL均能刺激SMC DNA合成,促进SMC增殖.

Oxidization-Modified Lipoproteins Stimulate DNA Synthesis of Cultured Human Arterial SMC

The arterial smooth muscle cells (SMC)are the predominant type of cells within atherosclerotic(As)lesions,and their proliferation plays an important role in the process of As genesis.On the basis of the establishment of primary culture and sub-culture method for human arterial SMC,the effects of LDL, VLDL,HDL,OX-LDL,OX-VLDL, OX-HDL on DNA synthesis of cultured human arterial SMC wereobserved.Results are as follows:(1) HDL had no stimulating effect on ³H-TdR incorporation into DNA of SMC (P>0.05),(2) LDL and VLDL showed the obvious stimulating effects (P<0.05);(3) OX-LDL, OX-VLDL and OX-HDL increased siginificantly ³H-TdR incorporation into SMC DNA, respectively (P<0.01). These results suggest that the atherogenic roles of LDL, VLDL, OX-LDL, OX-VLDL, OX-HDL are closely related to their stimulating effects on DNA synthesis and the proliferation of the arterial SMCs.