| 重组尿激酶原的纯化和性质研究 |
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| 引用本文： | 叶建新,肖成祖,张正光.重组尿激酶原的纯化和性质研究[J].生物化学与生物物理进展,1996,23(1):63-65. |
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| 作者姓名： | 叶建新 肖成祖 张正光 |
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| 作者单位： | 军事医学科学院生物工程研究所;军事医学科学院生物工程研究所;军事医学科学院生物工程研究所 |
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| 摘 要： | CHO工程细胞11G持续表达的pro-UK分泌在细胞培养液的上清中，培养液上清经过微孔玻璃(MPG)吸附色谱，羧甲基阳离子交换色谱，高压液相凝胶色谱三步纯化，纯化倍数可达700倍以上，总回收率为46％．再经过Benzamidine-Sepharose 6B亲和层析去掉少量的双链尿激酶，得到纯化尿激酶原．终产物经SDS-PAGE银染分析，纯度达90％以上，分子量为52 ku，其比活性为51 220 U/mg．抗体中和、二异丙基氟磷酸(DFP)抑制等实验证明重组pro-UK的性质和天然pro-UK的性质相一致．
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| 关 键 词： | 尿激酶原 纯化 微孔玻璃 Benzamidine－Sepharose 6B |
| 收稿时间： | 1995/2/20 0:00:00 |
| 修稿时间： | 1995/7/13 0:00:00 |
Purification and Characterization of a Recombinant Pro-urokinase |
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| Ye Jianxin,Xiao Chengzu and Zhang Zhengguang.Purification and Characterization of a Recombinant Pro-urokinase[J].Progress In Biochemistry and Biophysics,1996,23(1):63-65. |
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| Authors: | Ye Jianxin Xiao Chengzu and Zhang Zhengguang |
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| Institution: | Institute of Biotechnology, Academy of Military Medical Science;Institute of Biotechnology, Academy of Military Medical Science;Institute of Biotechnology, Academy of Military Medical Science |
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| Abstract: | The recombinant pro-urokinase expressed in CHO cells was purified. The purification procedure was based on the following steps: micro-pore glass chromatography, CM-15HR chromatography, Sephacryl S-200 gel filtration. The yield was 46% and the purification factor was 700-fold. Small amounts of contaminating urokinase were removed by Benzamidine-Sepharose 6B affinity chromatography. Analysis by SDS-PAGE showed that the purity of pro-urokinase was 90% and the molecular weight was 52 ku.The specific activity of the purified pro-UK was 51 220 U/mg of protein. The characters of the recombinant pro-UK were consistent with that of the natural pro-UK. |
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| Keywords: | pro-urokinase purification micro-pore glass Benzamidine-Sepharose 6B |
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