Effects of Protonophores on Membrane Electrical Characteristics in NG108-15 Cells |
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Authors: | Doebler Jeffrey A. |
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Affiliation: | (1) Neurotoxicology Branch, Pharmacology Division, US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD, 21010 |
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Abstract: | Studies were conducted to determine the effects of bath application of the protonophores carbonyl cyanide m-chlorophenylhydrazone (CCCP) and carbonyl cyanide p-(trifluoromethoxy)-phenylhydrazone (FCCP) on membrane electrical characteristics of differentiated NG108-15 (neuroblastoma X glioma hybrid) cells. Membrane resting potential (Vm), input resistance (Rin) and electrically induced action potential generation were measured using intracellular micro-electrode techniques. Both compounds produced concentration-dependent depolarization rather than the hyperpolarization commonly found with other central mammalian neurons. CCCP and FCCP also reduced Rin and disrupted the generation of action potentials in a concentration-dependent manner. The contribution of the observed alterations to the in vivo toxicity of these compounds remains to be established. |
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Keywords: | Action potential CCCP FCCP In vitro model membrane potential membrane resistance NG108-15 (neuroblastoma X glioma) hybrid cells |
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