Buoyant density centrifugation of sea urchin embryo chromatin on sucrose-glucose gradient |
| |
Authors: | Ševaljević Ljiljana Krtolica Koviljka |
| |
Institution: | 1. Institute for Biological Research, Belgrade, Yugoslavia
|
| |
Abstract: | Unsheard chromatin isolated from sea urchin embryos was submitted to buoyant density centrifugation in sucrose-glucose gradients. The main peak of blastula chromatin was at a density position of 1.299±0.028±0.009 g ml-1 whereas at gastrula stage a shift to a lower buoyant density position of (1.276±0.021±0.007 g ml-1) was observed. Besides the main peak, a small band with a density of 1.18 g ml-1 was noticed. The lighter fraction differed from the heavy one in a higher histone to DNA ratio, a lower proportion of the F-1 histone, and a lower nonhistone to DNA ratio. The most pronounced developmental alterations of proteins were observed at the level of nonhistone protein patterns of the light fractions. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |