Electroimmobilization of proinsulin C-peptide to a quartz crystal microbalance sensor chip for protein affinity purification |
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Authors: | Melles Ermias Anderson Henrik Wallinder Daniel Shafqat Jawed Bergman Tomas Aastrup Teodor Jörnvall Hans |
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Institution: | Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden. |
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Abstract: | Proinsulin C-peptide was electroimmobilized to a quartz crystal microbalance sensor chip, localizing this low-pI peptide for covalent attachment to activated surface carboxyl groups. The resulting chip was used in a continuous flow biosensor to capture anti-C-peptide antibodies, which could subsequently be eluted in 5% formic acid between air bubbles for efficient recovery and mass spectrometric identification. The method is reproducible through repeated cycles, providing affinity purification of proteins under real-time monitoring of the binding and elution processes. |
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Keywords: | Electroimmobilization Quartz crystal microbalance chip Biosensor Proinsulin C-peptide Mass spectrometry |
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