Variation in S phase in synchronous human cell lines |
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| Authors: | Leon N Kapp Dr Albert J T Millis Donald A Pious |
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| Institution: | (1) Laboratory of Radiobiology, University of California, 94143 San Francisco, California;(2) Department of Biological Sciences, The University at Albany, State University of New York, 12222 Albany, New York;(3) Department of Pediatrics and Genetics, University of Washington School of Medicine, 98195 Seattle, Washington;(4) Present address: Department of Pediatrics of the Albany Medical College, 12208 Albany, New York |
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| Abstract: | Summary Growth parameters of diploid and trisomic human fibroblasts were determined. The rate of growth of both classes of cells was
examined in asynchronous cultures, and diploid and trisomic cells had similar growth rates. Synchronous cultures were developed
using simple mitotic selection. The patterns and length of the DNA synthetic period (S phase) were found to be altered in
trisomy 21 cells when compared to diploid human or to heteroploid HeLa cells. Early S-phase synthesis was absent or reduced
and the overall length of the S phase was extended. However, the trisomic cells have apparently normal rates of DNA chain
elongation and normal replicon sizes.
This work was supported by the United States Department of Energy and the National Institutes of Health. |
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| Keywords: | trisomy 21 synchronous cells cell cycle S phase DNA synthesis |
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