Development and use of novel SSR markers for molecular genetic diversity in Italian millet (Setaria italica L.) |
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Authors: | Weiguo Zhao Gi-An Lee Soon-Wook Kwon Kyung-Ho Ma Myung-Chul Lee Yong-Jin Park |
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Institution: | 1. Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, 340-802, Korea 2. Jiangsu University of Science and technology, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu, 212018, China 3. National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, Suwon, 441-707, Korea 4. Legume Bio-Resource Center of Green Manure, Kongju National University, Yesan, 340-802, Korea
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Abstract: | Italian millet is a commercially important grain crop. Nineteen polymorphic simple sequence repeat (SSR) markers, developed through construction of an SSR-enriched library from genomic DNA of Italian millet (Setaria italica L., P. Beauv.), were used for assessment of molecular genetic diversity against 40 accessions of S. italica. In total, 85 alleles were detected, with an average of 4.5 alleles per locus. The average gene diversity and polymorphism information content (PIC) values were 0.412 and 0.376, ranging from 0.02 to 0.88 and from 0.02 to 0.87, respectively. Values for observed (H O) and expected (H E) heterozygosities ranged from 0 to 0.73 and from 0.03 to 0.89, respectively. Nine loci deviated from Hardy-Weinberg equilibrium. The mean similarity coefficient among accessions was 0.6593. Based on the UPGMA algorithm, six different groups were successfully identified. In this clustering analysis, all Korean accessions grouped in one cluster, indicating that Korean accessions are genetically quite distinct from other introduced accessions. These newly developed microsatellite markers should be very useful tools for several genetic studies, including an assessment of diversity and population structure in Italian millet. |
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