|
|||||
|
|
Production and purification of lipase by a mutant strain ofRhizopus arrhizus |
|
| Authors: | M Chattopadhyay A K Banik S Raychaudhuri |
| Institution: | (1) Department of Chemical Engineering, Biochemical Engineering Division, University of Calcutta, 700 009 Calcutta, India;(2) Department of Biophysics, Molecular Biology and Genetics, University of Calcutta, 700 009 Calcutta, India |
| Abstract: | Extracellular lipase production byRhizopus arrhizus was increased by mutant selection from 130 to 670 μmol FFA per mL per min using UV radiation and aziridine treatment. The produced lipase was purified 720-fold by ammonium sulfate fractionation and Sephadex G-100 gel filtration. The molar mass of the produced lipase was determined to be approximately 67 kDa which comigrated with bovine serum albumin in both a Sephadex G-100 column and SDS-PAGE. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |