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Leakage of recombinant penicillin G acylase from Escherichia coli by adding chloroform under fermentation conditions |
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| Authors: | Yongmei Jiang Dongzhi Wei |
| Affiliation: | (1) State Key Laboratory of Bioreactor Engineering, Institute of Newworld Biotechnology, East China University of Science and Technology, P.O.Box 311, Shanghai, 200237, China;(2) College of Life Science, Fujian Nomal University, Fuzhou, 350007, China |
| Abstract: | A simple method was developed to release periplasmic penicillin G acylase from Escherichia coli BL21(DE3) during the fermentation process. More than 80% of the total penicillin G acylase was released into the broth when 3% (v/v) chloroform was added at 3 h after induction. The activity of extracellular penicillin G acylase reached 20699 U/l. This method was efficient and would facilitate further investigation of penicillin G acylase for industrial applications. |
| Keywords: | Chloroform Escherichia coli Leakage Penicillin G acylase |
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