Metabolic profiling as a tool for prioritizing antimicrobial compounds |
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Authors: | Changsheng Wu Young Hae Choi Gilles P. van Wezel |
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Affiliation: | 1.Molecular Biotechnology, Institute of Biology,Leiden University,Leiden,The Netherlands;2.Natural Products Laboratory, Institute of Biology,Leiden University,Leiden,The Netherlands;3.Department of Microbial Ecology,Netherlands Institute of Ecology (NIOO-KNAW),Wageningen,The Netherlands |
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Abstract: | Metabolomics is an analytical technique that allows scientists to globally profile low molecular weight metabolites between samples in a medium- or high-throughput environment. Different biological samples are statistically analyzed and correlated to a bioactivity of interest, highlighting differentially produced compounds as potential biomarkers. Here, we review NMR- and MS-based metabolomics as technologies to facilitate the identification of novel antimicrobial natural products from microbial sources. Approaches to elicit the production of poorly expressed (cryptic) molecules are thereby a key to allow statistical analysis of samples to identify bioactive markers, while connection of compounds to their biosynthetic gene cluster is a determining step in elucidating the biosynthetic pathway and allows downstream process optimization and upscaling. The review focuses on approaches built around NMR-based metabolomics, which enables efficient dereplication and guided fractionation of (antimicrobial) compounds. |
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