Isolation and structure determination of a new lantibiotic cinnamycin B from Actinomadura atramentaria based on genome mining |
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| Authors: | Shinya Kodani Hisayuki Komaki Sho Ishimura Hikaru Hemmi Mayumi Ohnishi-Kameyama |
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| Affiliation: | 1.College of Agriculture, Academic Institute,Shizuoka University,Shizuoka,Japan;2.Graduate School of Integrated Science and Technology,Shizuoka University,Shizuoka,Japan;3.Graduate School of Science and Technology,Shizuoka University,Shizuoka,Japan;4.Biological Resource Center,National Institute of Technology and Evaluation (NBRC),Chiba,Japan;5.Food Research Institute,NARO,Ibaraki,Japan |
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| Abstract: | New lantibiotic cinnamycin B was isolated from the extract of Actinomadura atramentaria NBRC 14695T, based on genome mining and chemical investigation. The partial structure of cinnamycin B was established by 2D NMR experiments, which indicated that cinnamycin B had same methyl lanthionine bridging pattern with cinnamycin. The reduction with NaBH4-NiCl2 afforded the reduced cinnamycin B, and MS/MS experiment indicated the presence of hydroxy asparatic acid in the molecule. Cinnamycin B showed an antibacterial activity against Streptomyces antibioticus with dosage of 5 μg (0.5μL, 10 mg/mL solution) at spot-on-lawn testing method. The gene cluster of cinnamycin B on the genome of A. atramentaria was identified and discussed in comparison with that of cinnamycin. |
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