Insights into the fluoride-resistant regulation mechanism of Acidithiobacillus ferrooxidans ATCC 23270 based on whole genome microarrays |
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| Authors: | Liyuan Ma Qian Li Li Shen Xue Feng Yunhua Xiao Jiemeng Tao Yili Liang Huaqun Yin Xueduan Liu |
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| Affiliation: | 1.School of Minerals Processing and Bioengineering, Central South University,Changsha,China;2.Key Laboratory of Biometallurgy,Ministry of Education,Changsha,China;3.School of Nuclear Resources Engineering, University of South China,Hengyang,China;4.Key Discipline Laboratory for National Defense of Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang,China |
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| Abstract: | Acidophilic microorganisms involved in uranium bioleaching are usually suppressed by dissolved fluoride ions, eventually leading to reduced leaching efficiency. However, little is known about the regulation mechanisms of microbial resistance to fluoride. In this study, the resistance of Acidithiobacillus ferrooxidans ATCC 23270 to fluoride was investigated by detecting bacterial growth fluctuations and ferrous or sulfur oxidation. To explore the regulation mechanism, a whole genome microarray was used to profile the genome-wide expression. The fluoride tolerance of A. ferrooxidans cultured in the presence of FeSO4 was better than that cultured with the S0 substrate. The differentially expressed gene categories closely related to fluoride tolerance included those involved in energy metabolism, cellular processes, protein synthesis, transport, the cell envelope, and binding proteins. This study highlights that the cellular ferrous oxidation ability was enhanced at the lower fluoride concentrations. An overview of the cellular regulation mechanisms of extremophiles to fluoride resistance is discussed. |
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