Ultrastructure of wheat seedling mitochondria under anoxia and postanoxia

Summary To characterize the cytoplasmic streaming in characean cells, tonoplast-free cells were prepared and the chemical composition of the cytoplasm was modified by intracellular perfusion. Perfusion media containing sulfate inhibited the cytoplasmic streaming. The extent of inhibition was dependent on the concentration of ATP: the lower the concentration of ATP, the stronger the inhibition. It was suggested that inhibition by sulfate is competitive with respect to ATP. The effects of other anions were also examined.Abbreviations ATP adenosine-5-triphosphate - ATP] ATP concentration - ADP adenosine-5-diphosphate - EGTA ethylene glycolbis(-aminoethyl ether)-N,N,N,N-tetraacetic acid - MES 2-(N-morforino) ethanesulfonic acid - PEP phosphoenol pyruvate - Tris tris(hydroxymethyl) aminomethane - v velocity of cytoplasmic streaming