Conjugation of polyamidoamine dendrimers on biodegradable microparticles for nonviral gene delivery |
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| Authors: | Zhang Xue-Qing Intra Janjira Salem Aliasger K |
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| Affiliation: | Division of Pharmaceutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA. |
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| Abstract: | We report on the preparation and characterization of poly(D, L-lactide-co-glycolide) (PLGA) microparticles with surface-conjugated polyamidoamine (PAMAM) dendrimers of varying generations. The buffering capacity and zeta-potential of the PLGA PAMAM microparticles increased with increasing generation level of the PAMAM dendrimer conjugated. Conjugation of the PAMAM dendrimer to the surface of the PLGA microparticle removed generation-dependent cytotoxicity in HEK293 and COS7 cell lines. PLGA PAMAM pDNA microparticles displayed similar cytotoxicity profiles to unmodified PLGA pDNA microparticles in COS7 cells. A generation three PAMAM dendrimer conjugated to PLGA microparticles significantly increased transfection efficiencies in comparison to unmodified PLGA microparticles. |
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