电针对大鼠脑内雌激素受体蛋白及其mRNA表达的影响

本文应用放射免疫分析（ＲＩＡ）、ＲＮＡ点杂交和Ｎｏｒｔｈｅｒｎ ｂｌｏｔ、单克隆抗体免疫组织化学和计算机图像处理技术研究电针对切除卵巢大鼠脑内雌激素受体（ＥＲ）蛋白和ｍＲＮＡ表达的影响。以探讨针刺作用的分子生物学机理。结果表明,切除卵巢可导致血雌二醇（Ｅ２）水平降低,动物脑内ＥＲ蛋白和ｍＲＮＡ的表达增强;电针实验穴位后,去卵巢大鼠血的Ｅ２含量明显增加,脑内ＥＲ蛋白和ｍＲＮＡ表达受到明显抑制。正常大

Effects of electroacupuncture on the expression of estrogen receptor protein and mRNA in rat brain

In order to show some light on the possible molecular mechanism of the effects ofacupuncture on the hypothalamic-pituitary-ovarian axial function,radioimmunoassay(RIA), RNA dot blot and Northern blot, monoclonal antibody immuno-histochemistryand computer image analysis technique were used to study the effects ofelectroacupuncture(EA) on the blood level of estradiol (E2) and the expression ofestrogen receptor protein and mRNA in the ovariectomized rat brain. The results show thatovariectomy induced a decrease of blood E2 level and increase of the expression ofestrogen receptor protein and mRNA in the brain. All these effects could be remarkablyreversed by previous EA treatment of ovariectomized rats. EA in intact rat, however, hadno effects on blood E2 and expression of estrogen receptor. The above results suggest thatEA at some effective acupoints may activate the production of body estrogen, resulting ina long term increase or suppression at the level of gene(s) expression and consequentnormalization on the dysfunction of hypothalamic-pituitary-ovarian axis.