Characterization of microsatellites and development of chromosome specific STMS markers in bread wheat |
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Authors: | Rajeev K Varshney Alok Kumar Harindra S Balyan Joy K Roy Manoj Prasad Pushpendra K Gupta |
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Institution: | (1) Molecular Biology Laboratory, Department of Agricultural Botany, Ch. Charan Singh University, 250 004 Meerut, U.P., India |
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Abstract: | Microsatellites, or simple sequence repeats (SSRs), have become the markers of choice for genetic studies with many crop species
including wheat. Currently an international effort is underway to enrich the repertoire of available sequence tagged microsatellite
site (STMS) markers in wheat. As a part of this effort, we have sequenced 43 clones obtained from a microsatellite-enriched
wheat genomic library; 34 clones contained 41 different microsatellites. These microsatellites (mono-, di-, tri- nucleotide
repeats) were classified as 19 simple perfect, 18 simple imperfect and 4 compound imperfect types. Dinucleotide repeats were
the most abundant (70%). Primer pairs for only 16 microsatellites could be designed, since the flanking sequences of the others
were either too short or were otherwise not suitable for designing the microsatellite specific primers. Microsatellite loci
of the expected size and polymorphism were successfully amplified from 15 of these 16 primer pairs using three wheat varieties.
14 loci detected by 12 out of the 15 functional primer pairs were assigned to 11 specific chromosomes.
An erratum to this article is available at . |
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Keywords: | Bread wheat chromosome assignment microsatellite molecular marker polymorphism |
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