粉葛主要营养物质、异黄酮成分的积累规律及其相关性分析

为确定粉葛的最佳采收时期及各部位的应用价值，探索粉葛主要营养物质与异黄酮成分的积累规律及相关关系，该研究以广西藤县粉葛为材料，采用测试盒法、酶重量法、索氏抽提法、高效液相色谱法等生理生化技术，测定了成熟期不同部位异黄酮成分的含量及不同生长时期粉葛块根主要营养物质、异黄酮成分的含量，并分析了各营养物质与异黄酮成分积累的相互关系。结果表明：(1)在成熟期，粉葛不同部位的染料木素含量差异均不显著，但叶片的大豆苷元含量显著小于中藤，根头和藤的总异黄酮、葛根素、大豆苷的含量显著大于叶片和块根，并且根头和藤的葛根素含量均超过1.00%。(2)不同生长时期，粉葛的淀粉、多糖、粗蛋白、可溶性蛋白的积累在11月和12月达到最大，不溶性膳食纤维的积累在12月最小，可溶性膳食纤维在8月和12月的积累量最大，总异黄酮和葛根素在8月和9月的积累量最大。(3)总异黄酮、葛根素的积累与淀粉、多糖的积累呈显著负相关，总异黄酮、葛根素的积累与不溶性膳食纤维的积累呈显著正相关。综上结果认为：粉葛的根头、藤蔓含有丰富的总异黄酮、葛根素、大豆苷，具有很好的药用开发价值；粉葛药用的最佳采收期为8月和9月，粉葛食用的最佳采收期为...

Accumulation rule of main nutrients and isoflavones and their relationships in Pueraria lobata var. thomsonii

In order to determine the optimal harvesting period and the application value of each part of Pueraria lobata var. thomsonii and to explore the correlation between the main nutrients and the isoflavones, P. lobata var. thomsonii of Tengxian, Guangxi was used as the material, the contents of isoflavones in different parts, the contents of main nutrients and isoflavones in different growth periods were determined by test box method, enzyme gravimetric method, Soxhlet extraction method, high performance liquid chromatography and other physiological and biochemical techniques, and the relationship between the accumulation of various nutrients and isoflavones was analyzed to clarify the accumulation rule of main nutrients and isoflavonoids and their relationships in P. lobata var. thomsonii. The results were as follows:(1)At the mature stage, the content of genistein in different parts of P. lobata var. thomsonii was not significantly different, but the content of isoflavoues aglycone in leaf was significantly lower than that in middle vine, and the contents of total isoflavones, puerarin and daidzein in root apex and vine were significantly higher than those in leaf and tuberon, and the contents of puerarin in root apex and vine were more than 1.00%.(2)The accumulation of starch, polysaccharide, crude protein, and soluble protein reached the maximum in November and December, the accumulation of insoluble dietary fiber reached the minimum in December, the accumulation of soluble dietary fiber was the maximum in August and December, and the accumulation of total isoflavones and puerarin reached the maximum in August and September.(3)The correlation analysis results showed that the accumulation of total isoflavones and puerarin was negatively correlated with the accumulation of starch and polysaccharide; the accumulation of total isoflavones and puerarin was positively correlated with the accumulation of insoluble dietary fiber. Therefore, it can be concluded that the root apex and vine of pueraria powder contain rich total isoflavones, puerarin, daidzein, which has good value for medicinal development; the best harvest time for medicinal using are August and September, and the best harvest time for edible using are November and December; the accumulation of total isoflavones and puerarin is related to starch, polysaccharide and insoluble dietary fiber. This study clarify the accumulation rule and correlation of the main nutrients and total isoflavones in P. lobata var. thomsonii, and provides a reference for the comprehensive development and utilization of P. lobata var. thomsonii and the determination of the harvest time.