Phosphoproteomics reveals a distinctive Mec1/ATR signaling response upon DNA end hyper‐resection |
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Authors: | Ethan J Sanford William J Comstock Vitor M Faa Stephanie C Vega Robert Gnügge Lorraine S Symington Marcus B Smolka |
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Institution: | 1. Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca NY, USA ; 2. Department of Biochemistry and Immunology and Cell‐Based Therapy Center, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto Brazil ; 3. Department of Microbiology and Immunology, Columbia University Irving Medical Center, New York NY, USA |
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Abstract: | The Mec1/ATR kinase is crucial for genome maintenance in response to a range of genotoxic insults, but it remains unclear how it promotes context‐dependent signaling and DNA repair. Using phosphoproteomic analyses, we uncovered a distinctive Mec1/ATR signaling response triggered by extensive nucleolytic processing (resection) of DNA ends. Budding yeast cells lacking Rad9, a checkpoint adaptor and an inhibitor of resection, exhibit a selective increase in Mec1‐dependent phosphorylation of proteins associated with single‐strand DNA (ssDNA) transactions, including the ssDNA‐binding protein Rfa2, the translocase/ubiquitin ligase Uls1, and the Sgs1‐Top3‐Rmi1 (STR) complex that regulates homologous recombination (HR). Extensive Mec1‐dependent phosphorylation of the STR complex, mostly on the Sgs1 helicase subunit, promotes an interaction between STR and the DNA repair scaffolding protein Dpb11. Fusion of Sgs1 to phosphopeptide‐binding domains of Dpb11 strongly impairs HR‐mediated repair, supporting a model whereby Mec1 signaling regulates STR upon hyper‐resection to influence recombination outcomes. Overall, the identification of a distinct Mec1 signaling response triggered by hyper‐resection highlights the multi‐faceted action of this kinase in the coordination of checkpoint signaling and HR‐mediated DNA repair. |
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Keywords: | Dpb11 homologous recombination Mec1 resection Sgs1 |
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