Successful micropropagation protocol of <Emphasis Type="Italic">Piper methysticum</Emphasis> |
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Authors: | Z Zhang L Zhao X Chen X Zheng |
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Institution: | (1) State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, 571101, P.R. China |
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Abstract: | An efficient in vitro propagation of kava (Piper methysticum) was established. Utilizing 15-d-old tender shoots from dormant auxiliary buds as explants, significant induction of vigorous
aseptic cluster shoots was achieved in Murashige and Skoog (MS) medium containing 0.5 mg dm−3 6-benzyladenine (BA), 0.5 mg dm−3 indole-3-acetic acid (IAA), and antibiotics after 30 d. In vitro rooting was achieved at 100 % efficiency in MS medium containing 0.75 to 1.00 mg dm−3 IAA or indole-3-butyric acid and 3 % sucrose. The most robust and long roots were observed in medium with IBA. Moreover,
the embryonic callus was induced from petioles in MS medium supplemented with 1.0 mg dm−3 BA and 0.1 mg dm−3 IAA, of which 70 % differentiated into shoots in the presence of 1.0 mg dm−3 BA and 0.5 mg dm−3 IAA. |
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Keywords: | callus cluster shoots dormant auxiliary bud endogenous contamination growth regulators |
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