The recombinant adeno-associated virus vector (rAAV2)-mediated apolipoprotein B mRNA-specific hammerhead ribozyme: a self-complementary AAV2 vector improves the gene expression |
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Authors: | Shumei?Zhong Shihua?Sun Email author" target="_blank">Ba-Bie?TengEmail author |
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Institution: | (1) Research Center for Human Genetics, Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, Houston, TX, 77030, U.S.A;(2) University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX, 77030, U.S.A |
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Abstract: | Background In humans, overproduction of apolipoprotein B (apoB) is positively associated with premature coronary artery diseases. To
reduce the levels of apoB mRNA, we have designed an apoB mRNA-specific hammerhead ribozyme targeted at nucleotide sequences
GUA6679 (RB15) mediated by adenovirus, which efficiently cleaves and decreases apoB mRNA by 80% in mouse liver and attenuates the
hyperlipidemic condition. In the current study, we used an adeno-associated virus vector, serotype 2 (AAV2) and a self-complementary
AAV2 vector (scAAV2) to demonstrate the effect of long-term tissue-specific gene expression of RB15 on the regulation apoB
mRNA in vivo. |
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