Glycosidases in organic solvents: II. Transgalactosylation catalysed by polyethylene glycol-modified β-galactosidase |
| |
Authors: | Jean E Beecher Anthony T Andrews Evgeni N Vulfson |
| |
Institution: | Department of Biotechnology and Enzymology, AFRC Institute of Food Research, Shinfield, Reading, UK |
| |
Abstract: | β-
-Galactoside galactohydrolase (E.C. 3.2.1.23) was chemically modified with 1,1′-carbonyldiimidazole-activated polyethylene glycols (MW 2,000, 8,000, and 20,000). The modified β-galactosidases had over 50% of amino groups coupled to polyethylene glycol but retained over 50% of the original activity. The hydrophobically modified enzymes were soluble in chlorinated organic solvents in which transferase activity has been demonstrated. The transferase activity, its dependency on water content, and the thermostability of all three modified enzymes were compared. |
| |
Keywords: | PEG-modification β-galactosidase Galactosylation in organic solvents |
本文献已被 ScienceDirect 等数据库收录! |
|