ATPase Activity of Latrotoxin-Like Protein

We investigated the ability of latrotoxin-like protein for ATP hydrolysis. It was shown that latrotoxin-like protein possesses ATPase activity, which increases with shift of pH toward alkaline values. A comparison of the enzymatic activity of latrotoxin-like protein at different pH values showed that the kinetic parameters of ATP hydrolysis depend on pH of the incubation medium. It is suggested that the characteristics of latrotoxin-like protein are similar to those of proteins from the AAA-ATPase family, which mediate fusion of cell membranes.