Influence of in vitro growth conditions on photosynthetic competence and survival rate of Rehmannia glutinosa plantlets during acclimatization period |
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Authors: | Seon Jeong-Hoon Cui Yong-Yi Kozai Toyoki Paek Kee-Yoeup |
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Institution: | (1) Present address: Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheong-ju, 361-763, South Korea;(2) Faculty of Horticulture, Chiba University, Matsudo, Chiba 271, Japan |
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Abstract: | The photosynthetic responses of Rehmannia glutinosa grown under photoautotrophic or heterotrophic conditions in vitro were investigated after transfer to greenhouse conditions. In addition, the changes in carbohydrate content and survival
rates of the plantlets were evaluated. During six days after transplantation, the photosynthetic rate declined and photoinhibitory
impairments represented by decrease of Fv/Fm and chlorophyll content were observed regardless of environmental conditions
in vitro. Excessive transpiration was observed in plantlets grown under heterotrophic conditions during that period. Fructose and
glucose content of the plantlets grown under photoautotrophic conditions increased with time and reached almost the same level
of field grown plants after day 15. Under heterotrophic conditions, in contrast, the content of these sugars decreased continuously
during that period. It is suggested that high survival rate of plantlets grown under photoautotrophic conditions has to be
attributed to improvement of photosynthetic competence by imposed high light intensity and CO2 concentration in vitro. The results strongly suggest that the control of transpiration during early stage after transplantation plays a key role
in the acclimatization process, and photoautotrophic conditions could be a solution to solve the problems associated with
transplantation stress.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | carbohydrate chlorophyll fluorescence photoautotrophic conditions photosynthetic rate survival rate |
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