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A 50-kilodalton Cry2A peptide is lethal to Bombyx mori and Lymantria dispar
Authors:Ohsawa Masataka  Tanaka Miki  Moriyama Kenta  Shimazu Mitsuaki  Asano Shin-ichiro  Miyamoto Kazuhisa  Haginoya Kohsuke  Mitsui Toshiaki  Kouya Tomoaki  Taniguchi Masayuki  Hori Hidetaka
Institution:Laboratory of Applied Biochemistry, Graduate School of Science and Technology, Niigata University, Japan.
Abstract:The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis, and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides killed the larvae of both insects.
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