An optimized two-finger archive for ZFN-mediated gene targeting |
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| Authors: | Gupta Ankit Christensen Ryan G Rayla Amy L Lakshmanan Abirami Stormo Gary D Wolfe Scot A |
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| Affiliation: | Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, USA. |
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| Abstract: | The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome. |
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