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Aspergillus niger DLFCC-90 rhamnoside hydrolase, a new type of flavonoid glycoside hydrolase |
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| Authors: | Liu Tingqiang Yu Hongshan Zhang Chunzhi Lu Mingchun Piao Yongzhe Ohba Masashi Tang Minqian Yuan Xiaodong Wei Shenghua Wang Kan Ma Anzhou Feng Xue Qin Siqing Mukai Chisato Tsuji Akira Jin Fengxie |
| Institution: | College of Biotechnology, Dalian Polytechnic University, China. |
| Abstract: | A novel rutin-α-L-rhamnosidase hydrolyzing α-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the α-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13. |
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