Structure and expression of a root-specific rice gene |
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| Authors: | B. Sylvia de Pater Rob A. Schilperoort |
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| Affiliation: | (1) Molbas Research Group, Department of Plant Molecular Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, Netherlands;(2) Present address: Botanical Laboratory, RUL/TNO Center for Phytotechnology, Nonnensteeg 3, 2311 VJ Leiden, Netherlands |
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| Abstract: | Two rice cDNA clones (COS6 and COS9) were isolated, corresponding to genes that were highly expressed in roots from seedlings and mature plants. A genomic clone (GOS9) corresponding to cDNA clone COS9 was isolated and the intron/exon structure was determined by comparing the nucleotide sequences of the mRNA and the genomic clone. 5 ends and 3 ends of the mRNA were determined by primer extension and S1-nuclease mapping respectively. The open reading frame present in GOS9 potentially encodes a protein (14kDa) that does not show any significant homology to other proteins in databases. |
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| Keywords: | rice root-specific gene expression |
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