Effect of medium composition and type of vessel closure on axillary shoot production of magnolia in vitro |
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Authors: | Aurelia Kamenicka Maria Lanakova |
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Institution: | (1) Institute of Forest Ecology, Slovak Academy of Sciences, Vieska nad Zitavou, 951 52 Slepcany, Slovak Republic |
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Abstract: | Proliferation of axillary shoots from nodal segments of saucer magnolia (Magnolia x soulangiana Soul.-Bod.) was achieved on modified Standardi and Catalano (S medium) and Lloyd and McCown (WPM) media containing 1.33 μmol·dm−3 BA and 0.54 μmol·dm−3 NAA. The greatest number of axillary shoots was produced on S-medium with full strength macronutrients. Statistically significant
were the differences in biomass of axillary shoots cultured in vessels sealed with plastic closures. Rooting of the shoots
was achieved on half strength S medium supplemented with 4.9, 9.8, 14.7 and 19.6 μmol·dm−3 IBA. Rooted plantlets were able to resume independent growth after a short period of acclimatization. |
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Keywords: | Magnolia x soulangiana Soul -Bod axillary shoots media composition media concentration type of vessel closure |
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