Comparison of ent-kaurene and ent-isokaurene synthesis in cell-free systems from etiolated shoots of normal and dwarf-5 maize seedlings |
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Authors: | Peter Hedden BO Phinney |
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Institution: | Department of Biology, University of California at Los Angeles, Los Angeles, CA 90024, U.S.A. |
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Abstract: | An active cell-free system, prepared from young etiolated shoots of normal Zea mays seedlings, was shown to biosynthesize the terpenoid hydrocarbons ent-kaur-16-ene, squalene and phytoene from mevalonic acid. The biosynthesis of ent-kaur-16-ene from mevalonic acid was compared using cell-free systems obtained from normal and dwarf-5 seedlings. ent-Kaur-16-ene was the predominant diterpene hydrocarbon synthesized by extracts from the normals; however, ent-kaur-15-ene was the major diterpene hydrocarbon synthesized by the dwarf-5 mutants. ent-Kaur-15-ene and ent-kaur-16-ene were also produced as minor products in the normal and dwarf-5 systems, respectively. The possible significance of the synthesis of the ‘wrong isomer’ (ent-kaur-15-ene) by the mutant is discussed. |
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Keywords: | cell-free extract |
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