Complex Formation of RNA Silencing Proteins in the Perinuclear Region of Neurospora crassa |
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| Authors: | Logan M. Decker Erin C. Boone Hua Xiao Benjamin S. Shanker Shannon F. Boone Shanika L. Kingston Seung A. Lee Thomas M. Hammond Patrick K. T. Shiu |
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| Affiliation: | *Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211;†Department of Biology, Barry University, Miami Shores, Florida 33161;‡School of Biological Sciences, Illinois State University, Normal, Illinois, 61790 |
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| Abstract: | In Neurospora, genes not paired during meiosis are targeted by meiotic silencing by unpaired DNA (MSUD). Here, our bimolecular fluorescence complementation (BiFC) study suggests that RNA-directed RNA polymerase, Dicer, Argonaute, and others form a silencing complex in the perinuclear region, with intimate interactions among the majority of them. We have also shown that SAD-2 is likely the anchor for this assembly. |
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| Keywords: | RNA interference Neurospora crassa meiotic silencing by unpaired DNA (MSUD) bimolecular fluorescence complementation (BiFC) fluorescent protein tagging |
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