Alterations in oviductal cilia morphology and reduced expression of axonemal dynein in diabetic NOD mice |
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| Institution: | 2. Cardiovascular Department, Guy’s and St Thomas’ NHS Foundation Trust, London, United Kingdom,;3. Maastricht University Medical Center, Cardiovascular Research Institute (CARIM), Maastricht, The Netherlands;1. Department of Dermatology, Venereology and Allergology of Medical Faculty of Leipzig University, Leipzig, Germany;2. Biotechnology Center, Dresden University of Technology, Dresden, Germany;3. Department of Dermatology, University of Cologne, Cologne, Germany;1. Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing 100029, China;2. Department of Physiology, Institute of Basic Medical Sciences and School of Basic Medicine, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100005, China;3. Center for Coronary Heart Disease, Cardiovascular Institute & Fu Wai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100037, China;1. Department of Cardiovascular Sciences, Campus Bio-Medico University, Rome, Italy;2. Cardiovascular Center Aalst, OLV Clinic, Aalst, Belgium |
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| Abstract: | In the present study, we examined the morphology of cilia and expression of the dynein intermediate chain 2 (DNAI2) in the oviduct of non-obese diabetic (NOD) mice. Results obtained with immunohistochemistry showed that DNAI2 expression was reduced in oviducts of diabetic NOD (dNOD) mice, as compared to that observed in the normoglycemic NOD (cNOD) group, especially in the acyclic dNOD mice. Oviductal cilia of dNOD mice appeared to be reduced in number. Results obtained with Western blot analysis revealed that the expression of DNAI2 protein was significantly less in oviducts of dNOD mice as compared to that of cNOD mice corroborating the results obtained with immunohistochemistry. Electron microscopic examination and quantitative imaging of thin sections of Epon-embedded oviducts of both dNOD and cNOD mice confirmed the reduction of the number of cilia in the oviduct of the dNOD group which also displayed aberrant axonemal ultrastructure, including disorganization of the axoneme and alteration of microtubule doublets into singlets as well as disruption of the plasma membrane in many cilia. Taken together, the present findings suggest that structural alterations of oviductal cilia in female diabetic NOD mice might be detrimental to the normal function of these particular cell structures in gamete transport. |
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| Keywords: | Oviductal cilia Axonemal dynein Type 1 diabetes Female NOD mice |
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