| Transglutaminase 3 expression in C57BL／6J mouse embryo epidermis and the correlation with its differentiation |
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| 作者姓名： | Zhang J Zhi HY Ding F Luo AP Liu ZH |
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| 作者单位： | [1]NationalLaboratoryofMolecularOncology,CancerInstitute,ChineseAcademyofMedicalSciences＆PekingUnionMedicalCollege,Beijing100021,China.／／LifeScienceInstitute,HebeiNormalUniversity,Shijiazhuang050016,China [2]NationalLaboratoryofMolecularOncology,CancerInstitute,ChineseAcademyofMedicalSciences＆PekingUnionMedicalCollege,Beijing100021,China. |
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| 摘 要： | Epidermal-type transglutaminase 3 (TGM3) is involved in the cross-linking of structural proteins to form the cornified envelope in the epidermis. In the present study, we detected the expression of TGM3 in the mouse embryo using RT-PCR.TGM3 mRNA is weakly presented from E11.5 to E14.5 and increases significantly from E15.5 to birth. Then we determined the spatial and temporal expression pattern of TGM3 in the skin and other organs by in situ hybridization. We found a deprivation of TGM3 in skin at E11.5, while a rich supply in periderm cells and a weak expression in basal cells from E12.5 to E14.5. From the period of E15.5 to E16.5, after keratinization in the epidermis, TGM3 was expressed in the granular and cornified layers. The electron microscopic observation of the C57BL/6J mouse limb bud skin development provided several morphological evidences for the epidermal differentiation. The above findings suggest that the expression of TGM3 plays a important role in the epidermis differentiation in embryogenesis.
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| 关 键 词： | 谷氨酰胺转移酶3 相关分析 表皮细胞 差异表达 C57BL／6J老鼠胚胎 |
Transglutaminase 3 expression in C57BL/6J mouse embryo epidermis and the correlation with its differentiation |
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| Zhang J,Zhi HY,Ding F,Luo AP,Liu ZH.Transglutaminase 3 expression in C57BL/6J mouse embryo epidermis and the correlation with its differentiation[J].Cell Research,2005,15(2):105-110. |
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| Authors: | Zhang Jian Zhi Hui Ying Ding Fang Luo Ai Ping Liu Zhi Hua |
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| Institution: | National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. |
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| Abstract: | Epidermal-type transglutaminase 3 (TGM3) is involved in the cross-linking of structural proteins to form the cornifiedenvelope in the epidermis. In the present study, we detected the expression of TGM3 in the mouse embryo using RT-PCR.TGM3 mRNA is weakly presented from E11.5 to E14.5 and increases significantly from E15.5 to birth. Then wedetermined the spatial and temporal expression pattern of TGM3 in the skin and other organs by in situ hybridization. Wefound a deprivation of TGM3 in skin at E11.5, while a rich supply in periderm cells and a weak expression in basal cellsfrom E12.5 to E14.5. From the period of E15.5 to E16.5, after keratinization in the epidermis, TGM3 was expressed inthe granular and cornified layers. The electron microscopic observation of the C57BL/6J mouse limb bud skin develop-ment provided several morphological evidences for the epidermal differentiation. The above findings suggest that theexpression of TGM3 plays a important role in the epidermis differentiation in embryogenesis. |
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| Keywords: | transglutaminase 3 epidermis differentiation C57BL/6J mouse embryo |
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