Isolation and characterization of microsatellite loci in Pedicularis verticillata L. using PCR-based isolation of microsatellite arrays (PIMA) |
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Authors: | Tsurng-Juhn Huang Yi-Yen Chen Yen-Ping Li Cheng-Yu Hung Tzen-Yuh Chiang Chang-Hung Chou |
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Affiliation: | (1) Research Center for Biodiversity and Graduate Institute of Ecology and Evolutionary Biology, China Medical University, No. 91 Hsueh-Shih Road, Taichung, 404, Taiwan;(2) Department of Life Sciences, Cheng Kung University, Tainan, 552, Taiwan |
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Abstract: | Pedicularis verticillata L. is a highly valuable herb for traditional Chinese medical treatment. In this report, 11 microsatellite loci from P. verticillata were isolated. The simple sequence repeat (SSR) markers were screened in 23 samples of wild populations of P. verticillata, and eight samples from its sister P. ikomai. The number of alleles ranged from 4 to 13, and value of expected (H E) and observed (H 0) heterozygosity was 0.62609–0.89662 and 0–0.95652, respectively. All loci were significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the restrictedly distributed species. The markers amplified well in the two species are useful for examining genetic diversity and population genetic structure, which, in turn, can provide information for establishing conservation strategy for these endangered species. |
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Keywords: | Microsatellite PIMA Pedicularis verticillata L. Heterozygosity |
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