Three-Dimensional Reconstruction of Rabbit-Derived Pneumocystis carinii from Serial-Thin Sections II: Intermediate Precyst |
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Authors: | FRANCOIS PALLUAULT BRUNO PIETRZYK EDUARDO DEI-CAS CHRISTIAN SLOMIANNY BENOIT SOULEZ DANIEL CAMUS |
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Affiliation: | I.N.S.E.R.M. (U. 42), Unitéde Biologie et Biochimie Parasitaires et Fongiques, domaine du C.E.R.T.I.A., 369, rue Jules-Guesde, Flers-Bourg, 59650 Villeneuve d'Ascq, France;Hautes Etudes Industrielles, 13, rue de Toul, 59800 Lille, France;Laboratoire de Parasitologie-Mycologie, Facultéde Médecine, 1, Place de Verdun, 59045 Lille, France |
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Abstract: | Three-dimensional reconstruction of a binucleate intermediate precyst of Pneumocystis carinii was performed from serial-thin sections using the CATIA (Conception Assistée Tridimensionnelle Inter Active) Dassault system program. The presence of a mitochondrion, complex well-developed endoplasmic structures, and numerous Golgi vesicles was established. A better understanding of the ultrastructure of rabbit-derived P. carinii stages made it possible to formulate hypotheses on the evolution and physiology of the endomembrane system. Thus, the presence of the well-developed endoplasmic saccular structure and more than 230 Golgi vesicles in its vicinity might be implicated in the differentiation of the parasite surface structures and might also be related to nuclear division and individualization of intracystic bodies. |
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Keywords: | Computer-aided reconstruction endomembrane system Golgi complex life cycle ultrastructure. |
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