Method for B-phycoerythrin purification from Porphyridium cruentum |
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| Authors: | A. A. Tcheruov K. M. Minkova D. I. Georgiev N. B. Houbavenska |
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| Affiliation: | (1) National Center of Infectious and Parasitic Diseases, 1504 Sofia, Bulgaria;(2) Institute of Plant Physiology !["ldquo"]("/content/v472838672p22923/xlarge8220.gif") M. Popov!["rdquo"]("/content/v472838672p22923/xlarge8221.gif") , Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria |
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| Abstract: | Summary Porphyridium cruentum extract was treated with rivanol for the precipitation and elimination of the polysaccharide typical for this alga, while all phycobiliproteins remained solubilized. After their precipitation with ammonium sulphate, B-phycoerythrin was differentially separated from the other phycobiliproteins, and rivanol was removed by Sephadex G-25 gel filtration. The purity of B-phycoerythrin was proved.Abbreviations B-PE B-phycoerythrin - b-PE b-phycoerythrin - R-PC R-phycocyanin - APC allophycocyanin - PBP phycobiliproteins |
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