Improved 4D NMR experiments for the assignment of backbone nuclei in13C/15N labelled proteins |
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| Authors: | Wayne Boucher Ernest D Laue Sharon L Campbell-Burk Peter J Domaille |
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| Institution: | (1) Cambridge Centre jor Molecular Recognition, Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW Cambridge, UK;(2) DuPont Merck Pharmaceutical Co, P.O. Box 80328, 19880-0328 Wilmington, DE, USA |
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| Abstract: | Summary We recently proposed a novel 4D NMR strategy for the assignment of backbone nuclei in13C/15N-labelled proteins (Boucher et al., 1992). Intra-residue (and many sequential) assignments are obtained from a HCANNH experiment, whereas sequential assignments are based on a complementary HCA(CO)NNH experiment. We present here new constant time 4D HCANNH, HCA(CO)NNH and HNCAHA experiments that are more sensitive. Some of the data were presented at the 33rd ENC held at Asilomar, California, U.S.A., in April 1992. |
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| Keywords: | 4D NMR Resonance assignment Backbone nucle Larger proteins Isotopic labelling c-H-ras p21 |
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