Surface Charge Density Estimation of Vigna mungo Protoplasts Using a Fluorescent Dye, 9-Aminoacridine |
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| Authors: | Oka, Kotaro Ikeshima, Hiroko Ishikawa, Hideo Ohta, Eiji Sakata, Makoto |
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| Affiliation: | 1Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University Kohokuku, Yokohama 223, Japan
2Department of Physics, School of Helath Sciences and Biomedical Engineering, Kyorin University Miyashita, Hachioji, 192, Japan |
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| Abstract: | We showed that the surface charge density of protoplasts canbe estimated by the 9-aminoacridine method. The estimated surfacecharge density of the protoplasts isolated from elongating regionsof Vigna mungo root was 39 ? 8 mC/m2. The negative surfacecharge density increased when protoplasts were treated withglutaraldehyde or when EDTA was added to the protoplast suspensionmedium. These results support the validity of our estimationof the surface charge density of protoplasts by the 9-aminoacridinemethod. The concentration of amino groups at the surface ofthe protoplasts was estimated to be 34 mC/m2. (Received June 19, 1987; Accepted April 11, 1988) |
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