Ion and Glycerol Concentrations in 12 Isolates of Dunaliella |
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Authors: | GINZBURG, MARGARET GINZBURG, BEN ZION |
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Abstract: | Ginzburg, M., and Ginzburg, B. Z., 1985. Ion and glycerol concentrationsin 12 isolates of Dunaliella.J. exp. Bot. 36: 10641074. Twelve isolates of Dunaliella with average cell volumes rangingfrom 50 to 1400x1018 m3 were grown in batch culture at0.5 M or 2.0 M NaCl. Glycerol and ions (Na+, K+, Mg2+, CI,phosphate) were measured in log-phase cultures. The contentsof Mg2+, K+ and phosphate per cell were found to be a functionof cell-volume. Cell glycerol, Na+ and Cl were functionsof cell-volume and of the NaCl concentration in the medium.Solute concentrations were calculated from the measured cell-volumesand from the 3H2O content of pellets corrected for intercellularspace using Blue Dextran. Cell glycerol was found to accountfor about one-half of the expected osmolarity, the remainderbeing largely accounted for by Na+ and CI. Key words: Dunaliella, isolates, glycerol, ion concentrations |
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