Efficient production of chicken egg yolk antibodies against a conserved mammalian protein |
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| Authors: | M Gassmann P Th?mmes T Weiser U Hübscher |
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| Institution: | Department of Pharmacology and Biochemistry, University of Zürich-Irchel, Switzerland. |
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| Abstract: | The egg yolk of immunized chicken is a rich and inexpensive source of specific polyclonal antibodies. In this paper we show that 20-30 micrograms of a highly conserved mammalian protein, as exemplified by proliferating cell nuclear antigen, are sufficient to induce an immune response. Immunoblot analysis revealed that specific antibodies appeared 20 days after immunization, reached a plateau after 30 days, and remained high until at least day 81. A total amount of 4 g immunoglobulin was extracted from 62 eggs of one immunized hen, yielding approximately 130 mg of specific antibodies. |
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