Unusual genetic phenomena associated with Tn5 mutagenesis in Alcaligenes eutrophus strain H1 |
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| Authors: | W.-Y. W. Chow J. B. Peterson A. G. Atherly |
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| Affiliation: | (1) Department of Genetics, Iowa State University, 50011 Ames, IA, USA;(2) Department of Botany, Iowa State University, 50011 Ames, IA, USA;(3) Present address: Institute of Molecular Biology, Academica Sinica, Nanking, 115-29 Taipei, Republic of China |
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| Abstract: | Tn5 was introduced into Alcaligenes eutrophus strain H1 by a suicide vector pSUP1011. Physical characterization of mutants obtained after Tn5 mutagenesis revealed a relatively high frequency of plasmid curing, or deletion of a 50 kb plasmid DNA segment. Results of Southern hybridization and chromosomal walking indicate that the same continuous stretch of plasmid DNA (designated as D region of plasmid) is deleted in four independent isolates. Moreover, the same deletion of plasmid DNA is also observed in a mitomycin C-generated mutant strain H1-4.Journal Paper No. J-12095 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa. Project No. 2607, supported in part by a grant from the Iowa High Technology Council |
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| Keywords: | Tn5 Alcaligenes eutrophus Genetic rearrangements Plasmid curing Hydrogenase |
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