| Abstract: | Using a pair of primers (Primer Ⅰ and Primer Ⅱ), the authors have amplified a fragment of ACC synthase gene about 1025 bp from four varieties of gynoecious species of cucumber （Cucumis sativus L.） viz.:“CORONA”,“DALEVE”,“Zhongnong No.5”,and “Ouzhou No.8”. Sequence analysis revealed that this fragment of ACC synthase gene was more than 99% homologous with the gene reported by Trebitsh et al (1997). The authors regard them as the same gene, but it exhibited less homology with this ACC synthase gene when expressed by other induction. Southern blot analysis showed that this fragment of ACC synthase gene is associated with the sexual phenotype of cucumber,and it is the specific gene of gynoecium. However, the number of its copies has no direct correlation with the degree of female expression; this seems to indicate that there might be other genes associated with the degree of feminization. |
