| Abstract: | Genetic diversity at Gli-1, Gli-2 and Glu-1 loci was investigated in 89 Sichuan wheat ( Triticum aestivum L.) landraces by using acid polyacrylamide gel electrophoresis (APAGE) and SDS-PAGE. In these landraces, a total of 32 gliadin and 3 high-molecular-weight (HMW) glutenin patterns were observed. In total, 14, 15 and 5 alleles were identified at Gli-1, Gli-2 and Glu-1, respectively. At each locus, the alleles in higher frequency were Gli-A1a (89%), Gli-B1 h (46%), Gli-D1a (65%), Gli-A2a (64%), Gli-B2j (45%), Gli-D2 a (48%), Glu-A1c (99%), Glu-B1b (99%) and Glu-D1a (100%). The Nei's genetic variation index (H) of Sichuan wheat landraces was 0.3706, varying from 0 to 0.7087. The highest genetic diversity was found at Gli-B2 locus, while the lowest was found at Glu-D1 . The genetic diversity at Gli loci was higher than that of Glu-1 loci among these landraces, but it was much lower than that of modern wheat cultivars. These results indicated a narrow genetic base of Sichuan wheat landraces. In this study, “Chengdu-guangtou” had the identical gliadin and HMW-glutenin patterns with “Chinese Spring”, further supporting the proposal that “Chinese Spring” is a strain of “Chengdu-guangtou”. |
