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Malignant epithelial cell strains cultured from BALB/c mouse lung adenoma
Authors:G J Smith  J M Bentel
Affiliation:1. Cancer Cell and Molecular Biology Group, Malaghan Institute of Medical Research, Wellington 6012, New Zealand;2. Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand;3. Molecular Therapy Group, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague 142 20, Czech Republic;4. Mitochondria, Apoptosis and Cancer Research Group, School of Medical Science and Griffith Health Institute, Griffith University, Southport, QLD 4222, Australia;1. Department of Earth and Planetary Sciences, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro, Tokyo 152-8551, Japan;2. Lunar and Planetary Institute, Universities Space Research Association, 3600 Bay Area Blvd., Houston, TX 77058, USA;3. Department of Terrestrial Magnetism, Carnegie Institution of Washington, 5241 Broad Branch Road, NW, Washington, DC 20015-1305, USA;4. Center for Isotope Cosmochemistry and Geochronology, Astromaterials Research & Exploration Science Directorate, Johnson Space Center, NASA, Mail Code KR111, 2101 NASA Parkway, Houston, TX 77058, USA;5. Astromaterials Research & Exploration Science Directorate, Johnson Space Center, NASA, Mail Code KR111, 2101 NASA Parkway, Houston, TX 77058, USA;1. Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Sciences, Shandong Normal University, 88 East Wenhua Road, Jinan, Shandong, 250014, China;2. Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China;1. School of Life Sciences, Jawaharlal Nehru University, New Delhi, India;2. Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, India
Abstract:Urethane-induced lung adenomas from adult BALB/c mice were explanted onto a plastic substratum and cultured in order to establish the epithelial cell strain NUL1. The cell strain exhibited a polygonal morphology with high nuclear to cytoplasmic ratio and osmiophilic lamellar bodies characteristic of lung adenoma cells. A reproducible large and small cell heterogeneity was preserved despite multiple cell cloning. NUL1 was malignant at all passage numbers tested exhibiting anchorage-independent growth and subcutaneous formation of carcinomas in immune-suppressed mice. The cell strain was diploid at low passage numbers and became pseudo-diploid with increasing passages.
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