Endothelia of Schlemm's canal and trabecular meshwork: distinct molecular, functional, and anatomic features |
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Authors: | Alvarado Jorge A Betanzos Abigail Franse-Carman Linda Chen Janet González-Mariscal Lorenza |
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Affiliation: | Department of Ophthalmology, University of California, San Francisco, California 94143-0730, USA. jalva@itsa.ucsf.edu |
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Abstract: | The purpose of this study was to compare human endothelial cells from Schlemm's canal (SCEs) and the trabecular meshwork (TMEs) in terms of ZO-1 isoform expression, hydraulic conductivity (HC) properties, and "giant" vacuole (GV) formation. The principal study methods were Western blot, RT-PCR, immunofluorescence, and perfusion chambers. Blot signals for +-and --isoforms were similar in SCEs but less intense for the +-relative to the --signal in TMEs. With the anti-+ antibody used at 1/50 dilution, binding occurred at cell borders of both cell types, but only to SCEs when used at a 1/200 dilution in vitro and in vivo. SCEs were more resistive than TMEs (HC = 0.66 vs. 1.32 µl·min-1·mmHg-1·cm-2; P < 0.001) when perfused from apex to base. When perfused in the other direction, SCEs were again more resistive (5.23 vs. 9.04 µl·min-1·mmHg-1·cm-2; P < 0.01). GV formation occurred only in SCEs as a function of flow direction, perfusion pressure, and time. We conclude that SCEs and TMEs have distinctive phenotypic properties involving their content of ZO-1 isoforms, barrier function, and GV formation. tight junctions; ZO-1; giant vacuoles; conductivity |
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