| Abstract: | A serum-free growth medium composed of MCDB-104 supplemented with platelet-derived growth factor (PDGF) (3 μg/ml), epidermal growth factor (EGF) (100 ng/ml), insulin (INS) (5 μg/ml), transferrin (TRS) (5 μg/ml), and dexamethasone (DEX) (55 ng/ml) supports the proliferation of WI38 cells at a rate and to a density similar to that of medium supplemented with 10% fetal bovine serum (FBS). EGF exerts its effect at moderate cell densities while PDGF appears to modulate cell proliferation at high densities. Cells seeded into EGF-, INS-, TRS-, and DEX-supplemented medium enter S phase approx. 3 h earlier than cells seeded into PDGF-, EGF-, INS-, TRS- and DEX-supplemented medium or FBS-supplemented medium. |
