蜂毒素自聚集过程中间态的荧光光谱研究

应用荧光光谱及其一阶导数谱对蜂毒素在ＮａＣｌ诱导下的自聚集进行了研究，伴随蜂毒素自聚集的发生其内源荧光发射峰的峰宽在过渡点附近出现展宽并呈现出极大值，用二态转换模型可以对荧光发射谱进行较好的拟合，提示蜂毒由单体向四聚体的转化过程中不存在稳定的中间态。

STUDY ON INTERMEDIATE STATE IN THE PROCESS OF MELITTIN SELF -ASSOCIATION BY FLUORESCENCE SPECTRA

Intrinsic fluoascent emission of melittin and its first derivative spectra were used forstudying of the self-association of melittin induced by NaCl. In the self-association process,the bandwidth of melittin fluorescence emission spectram was broadered and reached a maximum at the transition point. It is found that the intrinsic emission spectrum containsmonomeric and tetrameric conponents by curve fitting analysis. The result indicates that themelittin self-association is consitent with two-state conversion model, and no homogeneousintermediate state exists during its self-association.