差示扫描量热法直接测定抗冻蛋白质溶液的热滞效应

文献上一直用显微镜观察法等测定抗冻蛋白的热滞效应，其冰晶量是靠观察晶核体积估算得得的，人为性很大，用并示扫描量热法直接测定沙冬青抗冻蛋白质溶液的热滞效应，通过熔融焓和冻结焓值准确地测定了冰晶含有量和热滞温度。同文献比较，沙冬青ＡＦＰ具有极高的抗冻活性，而且开创了一个新的有效的测量抗冻蛋白抗冻活性的途径。

DIRECT MEASUREMENT OF THERMAL HYSTERESIS EFFECT OF ANTINFREEZE PROTEIN SOLUTION BY DIFFERENTIAL SCANNING CALORIMETRY

The direct micrescopic observation has been used to measure the thermal hysteresis effects of antifreeze proteins in the litelature. The amount of ice Crystals in the system is roughly estimated by the obserVed volume of ice nudei, and thus it is much more artffidal. Here we report a dired differential scanning calorimetric measUrement of the thermal hysteresis effete of antifreeze protein sulution from arnmopiptanthus mongolicus. The thennal hystersis temperature and amount of ice crystal nuclei are quantitatively measured from DSC thennograms, melting and freezing enthalpies. Compared to the results reported in the literature, this antifreeze protein shows 2 much higher antifreeze activity and thus a new and accurate proedure to measure the aCtivity of antitreeze protein solution is provided.