Large scale deletions in the <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> genome create strains with altered regulation of carbon metabolism |
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Authors: | Kiriko Murakami Eriko Tao Yuki Ito Minetaka Sugiyama Yoshinobu Kaneko Satoshi Harashima Takahiro Sumiya Atsushi Nakamura Masafumi Nishizawa |
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Institution: | (1) Department of Microbiology and Immunology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan;(2) Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan;(3) RIISE, Hiroshima University, Hiroshima, Japan |
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Abstract: | Saccharomyces cerevisiae, for centuries the yeast that has been the workhorse for the fermentative production of ethanol, is now also a model system
for biological research. The recent development of chromosome-splitting techniques has enabled the manipulation of the yeast
genome on a large scale, and this has allowed us to explore questions with both biological and industrial relevance, the number
of genes required for growth and the genome organization responsible for the ethanol production. To approach these questions,
we successively deleted portions of the yeast genome and constructed a mutant that had lost about 5% of the genome and that
gave an increased yield of ethanol and glycerol while showing levels of resistance to various stresses nearly equivalent to
those of the parental strain. Further systematic deletion could lead to the formation of a eukaryotic cell with a minimum
set of genes exhibiting appropriately altered regulation for enhanced metabolite production.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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